Description
Aviva Systems Biology Heparin/Platelet Factor 4 antibody ELISA Kit (Human) (OKCD01299) is based on standard reverse capture sandwich enzyme-linked immuno-sorbent assay technology. A Heparin/Platelet Factor 4 antibody has been pre-coated and blocked in a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. An HRP conjugated detector antibody specific for Human antibody is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample HPF4 Antibody captured in well